In middle aged male rats, neutering was not found to result in osteoporosis.
In middle aged female rats, spaying resulted in the development of osteoporosis.
Link to (summary) abstract only.study confirmed that middle-aged female rats on a normal diet develop osteoporosis after castration; similar findings have been made by Saville (1969) and Kalu (1983)
https://pubmed.ncbi.nlm.nih.gov/3962636/